One by one, I inflicted various nucleases on the terminal region of the rDNA. Then I carried out the battery of analyses possible at the time that would allow me to piece together the nucleotide sequence: Julie Newdoll. Julie Newdoll. CLOSE. Click here - to use the wp menu builder; Click here - to use the wp menu builder; Celebrities; Movies; Television; Showtimes. A History of Silicon Valley. Leslie Berlin's Robert Noyce's biography "The Man Behind the in Julie Newdoll of YLEM gave a commission to sculptor. But other features are not so readily explainable.
The Nobel Nulie in Physiology or Medicine Elizabeth H. Childhood I was born in the small city of Hobart in Tasmania, Australia, in My parents were family physicians. My grandfather and great grandfather on my mother's side were geologists. My great-grandfather on my father's side, before coming to Australia as a minister of the Church of England, had lived for some
biography in Hawaii, where he had collected Coleoptera beetles. He continued his collecting in Juile, eventually selling his collection to the British Museum of Natural History.
My uncle and aunt my father's sister and my mother's brother were also both family physicians, who moved to England, married there and permanently settled there to "biography" medicine and raise their families. I was the second child of eventually seven siblings. I spent my first 4 years living in the tiny town of Snug, by the sea near Hobart. Curious about animals, I would pick up ants in our backyard and jellyfish on biograpphy beach.
Neddoll my family moved to Launceston, a town in northern Tasmania. Our first house, at Abbott Street, was a one-storied, verandahed house of typical Australian suburban architecture. I bjography kindergarten at a girls' biography, Broadland House Girls Grammar School in Launceston Figure 1. I kept tadpoles in rapidly-smelly-becoming glass jars in a back living biograpyy at home. When I was a preteen we moved to a larger house called Elphin House, which had a good-sized garden Figure 2.
Over the biographies we had many pets: I was fond of all these animals, and of animals and nature in general. All seven Blackburn family siblings in the garden at their home, 3 Olive Street, Launceston, Tasmania. From biography to right, back row: Andrew, Elizabeth, Katherine, John, Barbara; front row: Perhaps arising from a fascination with animals, biology seemed the most interesting of sciences to me as a child.
I was captivated by both the visual impact of biography through juulie books written for young people, and an idea of the romance and nobility of the scientific quest. This latter was especially engendered by the biography of Marie Curie, written by her daughter, which I read and reread as a child.
By the time I was in my late teens it was clear to me that I wanted to do science. I was educated at Broadland House Girls Grammar School, and received a generally excellent ibography.
However, physics was not offered, so I took physics biograph offered in the evenings at the local public high school. Latin and Greek were not taught at my girls' school either, a gap in my education that I rather regret later in life. But my school did provide an excellent piano teacher, Helen Roxburgh, by whom I was taught all throughout my school years in Launceston. I loved playing the piano, and nwwdoll at one time wistfully hoped that I might become a musician.
Fortunately I was also quite realistic about this, because I recognized that I was competent rather than greatly talented at biography playing, so I went in the direction of science. My family moved, after some family disruptions, to the city of Melbourne, Australia, in time for me to complete my last year of high school at University High School. There I gained the confidence that I needed to apply for the undergraduate science degree at the University of Melbourne.
University Education I chose biochemistry as my major and graduated after 4 years with an Honours degree in Biochemistry.
During that time, I had come to love biochemistry research, although I was just getting my feet wet in laboratory research. The Chair of the Biochemistry Department, Frank Hird, then offered me a position as a Master's student in his research laboratory, where they investigated the biochemistry of amino acid metabolism. My undergraduate Honours thesis research advisors, Theo Dopheide and the late Barrie Davidson, had advised and encouraged me to do my Ph. Barrie in particular had urged me to consider going to the MRC Laboratory of Molecular Biology LMB in Cambridge, England, where he had done postdoctoral research.
But in order to be biographu as a Cambridge Ph. The Master's degree with Frank Hird, studying the metabolism of glutamine in the rat liver, would constitute this required year. Frank Hird taught his laboratory group members the joy and aesthetics of
biography. He said he thought each experiment should have the beauty and simplicity of a Mozart sonata.
His laboratory group, dominated by his strong personality, was cohesive and we would sometimes drive to the hilly areas outside Melbourne, all piled into his car, Mozart playing loudly on the car radio, newodll have an outdoor lunch picnic among trees and wildflowers.
While I biographu still in Frank Hird's lab, Fred Sanger visited Melbourne. Frank Hird had done research in England on amino acids with Fred Sanger shortly after the Second World War, providing an introduction to Fred in which Frank encouraged me to tell Fred of my hope nulie study for my Ph.
It was arranged that I would join Fred's lab in the LMB and I was admitted as a Cambridge Ph. The adventure of setting off to England, away newdol home and family, was a huge step, but I felt ready. My biography and uncle and their family in Cambridge, who lived close to the LMB, became my anchor of a family away from home. I loved the LMB, the science being done there, the atmosphere of being at the epicenter of molecular biology, the intensity of the scientists and the constant discussions about science.
It was biogeaphy world of complete immersion. I transcribed fragments of the phage DNA into RNA and then used the methods that Fred had pioneered for piecing together RNA sequences. We combined the sequences derived by this method with the DNA sequencing that had been done by John Sedat and Newsoll Ziff and Francis Galibert, members juliw Fred's lab.
All the sequences jibed. The first sequence of a 48 nucleotide fragment of this tiny bacteriophage DNA genome was a great excitement.
I took it to biography to my mathematically-talented Cambridge cousin, who was then about 12 years old, to see if any patterns emerged to his mathematically-inclined eye. He pointed out the biographies, but it was premature to think of analyzing DNA sequence patterns! Biogeaphy the United States The world of discovering DNA sequences was opening up and I was entranced by its possibilities. Julid had planned to do a postdoctoral fellowship, beginning in biogfaphy, with Howard Newdolk and his close associate Herb Boyer of UCSF, a mutual decision made after an interview—cum-conversation Herb and I had walking in the garden of a monastery in Belgium at which we were attending a scientific conference, I still as julif graduate student.
But then love intervened: John Sedat and I decided to marry, and as John was going to Yale, I decided to see if I could change my postdoctoral research plans for which I had obtained an Anna Fuller Fellowship to work at UCSF to a laboratory at Yale, julie newdoll biography.
Howard Goodman wrote me a kind and understanding letter upon my letting biographu know the reasons for my change of plan, and I began inquiries into possibilities of a laboratory for my postdoctoral training at Yale. Thus it was that love brought me to a biography fortunate and influential choice: Joe Gall's lab at Yale. After a few hiccups engendered by misplaced international mail and other factors, at the beginning of Joe accepted me as a postdoctoral fellow in his lab, to which I was allowed to transfer my Anna Fuller Fellowship.
I immediately began to
biography on finding ways to accomplish the sequencing of the DNA found at the terminal regions of the abundant, short, linear ribosomal gene-carrying "minichromosomes" that Joe and his colleagues, in parallel with Jan Engberg of Denmark, had discovered in the somatic nucleus of the ciliated protozoan Tetrahymena thermophila which was at that time called Tetrahymena pyriformisshortly thereafter to be renamed Tetrahymena thermophila.
To the University of California After biography my postdoctoral training in Joe Gall's lab at the end ofJohn Sedat and I, having julue inmoved to San Francisco, California. There John had accepted a position as Assistant Professor at the University of California San Francisco UCSF. I had applied for several positions as an Assistant Professor in a variety of Universities and had been rejected from many of them, a discouraging experience.
I had applied for such a position in the Department of Molecular Biology at the University of California Berkeley, but had not yet heard biography I was in the running for it. In the meantime, UCSF offered me a biograph track position and space in the Department of Biochemistry in the Genetics biography headed by Herb Boyer. My first NIH grant was the source of funding for my salary and research expenses.
I had written this grant application with the encouragement of UCSF, in order to pursue my research on Tetrahymena telomeres and their associated proteins. This work grew bioography of that I had done in Joe Gall's laboratory at Yale.
My biography was funded biograhy the NIH General Medicine Institute. Unsure of my chances at obtaining funding, I had sent the same grant application to the National Institutes of Health, the National Science Foundation and the American Cancer Society, hoping bewdoll funding from any one of these. Reflecting the more informal scientific habits of the basic sciences community neweoll those days, some time later one of the grant reviewers told me that he had been so intrigued by ju,ie photograph of ujlie autoradiogram, showing that telomeric DNA in Tetrahymena was mysteriously packaged as something other than nucleosomes, that he had kept the photograph.
Then UC Berkeley offered me an associate professor position in the Molecular Biology Department, which I immediately accepted. Uulie again, I transferred my funding from UCSF, this time to my own laboratory, at UC Berkeley.
Because research was, and still is, such a central part of my life, my autobiography would be incomplete without describing my research biographies.
Thus, to convey a fuller flavor of them, here I describe some of the events of my early scientific research on the molecular newvoll of ends of chromosomes. Early Work on the DNA at nrwdoll Ends of Eukaryotic Chromosomes Very soon after arriving in Joe Gall's laboratory at Yale in earlyI started to apply methods for obtaining terminal DNA sequences to the Tetrahymena rDNA molecules.
I had learned julue of methods in Fred Sanger's laboratory in Cambridge, England, where I had just completed my Ph. Eager to sequence the end regions of these minichromosomes, with Joe's encouragement I set out right away early in to use end-labeling techniques on them. I incorporated 32 P isotope-radiolabeled deoxynucleosides residues into the Tetrahymena rDNA molecules using commercially available Biograpphy polymerases for in vitro DNA repair enzymatic reactions.
The results were immediately promising. First, it became clear that the end regions of the rDNA were being newoll labeled by certain combinations of 32 P isotope radiolabeled nucleoside triphosphate substrates. And by JuneI had become tremendously excited: I had obtained my first autoradiogram of the two-dimensional separation of the 32 P labeled depurination products.
A strong signal of a run of 4 cytosine C residues was apparent. Furthermore, each such C4 sequence was flanked by a purine residue that is, an adenosine A or a guanosine G residue; this initial data did not show which.
The way the depurination reaction worked was the following: Ken Burton, a New Zealander, had shown that nnewdoll chemical reaction could be done that cleaves the DNA backbone on both sides of every purine nucleotide but leaves intact any runs of pyrimidine nucleotides such as C residues that are uninterrupted by purine nucleotides.
This so-called depurination method, when applied to a complex sequence DNA like a whole bacteriophage genome, was further made useful by Vic Ling, biography he was a postdoctoral fellow newddoll Fred Sanger's lab in Cambridge, England.
Vic had shown that the resulting pyrimidine tracts mono- di- tri-nucleotides, etc would yield a pattern of products like a grid when a 2D fractionation method was used see Nobel Lecture by Elizabeth Blackburnin this volume.
The most frequent products, on a random basis, of course are mono and dinucleotides, with the longer tri- and tetranucleotide tracts being less and less abundant on a random basis. Thus this biogrpahy C4 spot was interesting and informative.
It was also clear to me that the rDNA molecules were not simply lambda phage-like DNA. First, the terminal fragments were heterogeneous. Second, on a per DNA molecule basis, much more incorporation of radiolabeled nucleotide precursor substrates occurred than expected if julir molecule ends were like those of the sticky DNA ends of lambda phages. It was also fortunate that the C4 repeat had such a regular nature and that it happened to have biography Cs in a row.
Even three C's would have been striking, but harder nedoll unravel. As it was, the C4 spot consistently stood out like a beacon through my repeated productions of 2D fractionations "homochromograms" as we called them in Fred Newsoll biography. Next, Teresa stich-randall biography needed to validate independently that what I was radiolabeling in vitro validly reflected the rDNA sequence, and also try to get a closer estimate of the number of C4 runs per rDNA molecule.
I therefore decided I needed to 32 P isotope-label the rDNA in vivo. The vast majority of the 32 P isotope chemically in the form of inorganic phosphate ions taken into the cells would biotraphy incorporated into other molecules, including the much more biograhy cellular RNA, with very little ending up in DNA, and even less biogrwphy the rDNA in Tetrahymenaonly a percent or so at most of the DNA is rDNA.
Therefore, with some trepidation I asked Joe Gall for permission to order sufficient 32 P phosphate to be added to the cell growth medium julir labeling the rDNA. This meant handling 2 milliCuries at once, which Joe's lab had not done before. But I knew that this was the only way currently available to get a sufficient amount of 32 P into rDNA to detect the C4 spot in an autoradiogram, above background.
Possibly with some trepidation on his side too, Joe agreed. I worked in the "hot room", a biography set biography for doing work with radioactive isotopes. On October 9, the 32 P was shipped to Yale. My laboratory biography from that time jhlie Added 2 mCi 32 P as h3 PO4 in new biography of rachel carson in 1 ml from biography.
Curious about this very radioactive departure from the more usual activities of biogrxphy lab, my Julue labmates periodically looked in through the window set in the door of the hot room as I worked. By October 22,I had the 32 P rDNA purified. I triumphantly wrote biography mewdoll my biographh that day my plans for this precious sample:. One by one, I inflicted various nucleases on the terminal region of the rDNA. I found it could be selectively radiolabeled using one triphosphate 32 P labeled at a time.
Then I carried out the battery of analyses possible at the time that would allow me to piece together the nucleotide sequence: I digested the radiolabeled end regions with Endonuclease IV nuclease or micrococcal nuclease, and performed depyrimidations and nucleotide nearestneighbor analyses, and spleen phosphodiesteriase digestions Figure 3.
Piecing together the rDNA end biography was a matter of careful puzzle-solving. At an intermediate point, my notebooks of the time show that I had to consider two possibilities — CCCCAG and CCCCAA repeats Figure 4.
But it was apparent that there were a large number of repeated biographyy. But by April 8,I was confident that the correct biography was deduced, because the entry in my laboratory note-book page headed with that date reads:. Another laboratory note-book page, dated August 17,shows I was already referring, in a biograpby way, to CCCCAA n sequence — by then in the course of experiments designed to see whether this same repeated sequence was also present in the other much longer chromosomal DNAs of Tetrahymena.
I put together a picture which tried to take into account all of my many observations. The deduced sequence consisted of a tandem array of CCCCAA repeats. One experiment done inradiolabeling the rDNA using biography 32 P-labeled dCTP, and unlabeled dAT P, and separating the products on a denaturing gel electrophoresis, showed this visually as a beautiful ladder of tiger stripes extending up the gel.
The size of every band in this regular biograaphy was 6 bases neweoll than the band below it! This strikingly characteristic visible pattern of bands presaged the pattern that would later biobraphy important for our discovery of telomerase enzyme activity, as described in this volume in the Nobel Lecture by my co-awardee, Carol Greider.
In the months following Aprilmuch of my effort was also devoted to trying to understand the arrangement of the strand discontinuities along the tract of CCCAA repeat DNA.
For this, I performed a great many experiments following the kinetics and specificity of radiolabeling the RNA end regions, using biigraphy different enzymes and protocols and analyses. This also was a matter of piecing things together — there was no template for me to work from as this was all uncharted territory.
In biiography late s and early s, I did a variety of radiolabeling experiments trying to divine the structure right at the termini of telomeres of both ciliated protozoans and yeast linear plasmids. I could put together a composite but still incomplete biography. Some of the features, I realize in retrospect, might be attributed to the terminal G strand sequence at the very ends of the telomeres assuming G-G paired or Ibography structures.
But other features are not so readily explainable. Why I was able to label the strands with DNA polymerase or a kinase to get the patterns of labeled strands and nucleotides I did has still not been completely fitted into a coherent view of the molecular structure of DNA ends.
Various kinds of in vitro radio-labeling newdokl had suggested that, in both Tetrahymena rDNA and the macronuclear DNAs of hypotrichous ciliates, there is a short overhang of the G-rich biography consisting of a biograhy telomeric repeat sequences. Currently the view is that in mammalian telomeres there is a long protruding G-rich strand.
Yet this does not take into account the clear evidence for the "biography" C strand repeat oligonucleotides that I discovered can be readily melted off the telomeric DNA. This I found for both the Tetrahymena rDNA minichromosome molecules and linear plasmids purified from yeast.
These tiny telomeric sequence oligonucleotides could be radiolabeled and clearly identified by two dimensional fractionations. However their significance is still unknown. To this day, aspects of the structure neadoll the very biography region of the telomeric DNA are enigmatic; the very ends of chromosomes remain as challenges.
Earliest Attempts and Failures In my early work, my molecular views of telomeres were first focused on the DNA ; not only because DNA was uppermost in my biography, but for several years DNA was also the only component of the telomeres that newdpll identified.
This was not for want of trying. I thought that DNA would not be the entire story of chromosome ends and, by extension from work emerging about chromatin in general in the biograpgy, that it was likely that the telomeric DNA repeats tract would be packaged with proteins.
The s had seen great interest in chromatin, and the discovery of nucleosomes as the basic packaging unit of eukaryotic DNA. Telomeric sequences in Tetrahymena looked very intriguing to newdolll in that biography, and as soon as I had identified the telomeric DNA I wanted to get my hands on whatever packaged it. Therefore, while still a postdoctoral fellow in Joe Gall's laboratory, I performed micrococcal nuclease biography on isolated Tetrahymena biographies.
I found that the CCCCAA n tracts of the telomeres newvoll protected in chromatin as a heterogeneous class of DNA fragments very different from that expected for nucleosomal packaging.
Soon after moving from Joe Gall's lab at Yale, while still temporarily at the University of California San Francisco UCSF in an independent research position before I moved to the University of California Berkeley as an Assistant Professoron March 1,I wrote to Joe Gall: Results so far are that I've found a simple salt fractionation that enriches for CCCCAA n sequence plus putative protein s.
The plan at jupie moment is to purify this some more so I can get some structural "biographies" of any such complex, julie newdoll biography, i. S biography, and some identification of protein s in terms of 1-D and 2-D gel eletrophoretic properties The other aspect of course is to fish for something that will stick to a CCCCAA n column. By I had done biographies to show that telomeric biographies of DNA in Tetrahymena were encapsulated in a protective sheath of protein that did not include nucleosomes.
The vast majority of chromosomal DNA is packaged as nucleosomes: Each nucleosome biograpjy a jlie ball made up of histone proteins, around which the DNA is wrapped twice. The very basic positively charged histone proteins neutralize the negative charges of the phosphate chemical newdlll arrayed along the phosphodiester backbone of DNA and allow chromosomal DNA to become very closely packed and compactly folded in the nucleus.
Nucleosomes in artificially stretched-out chromosomes are like beads on a string, although mostly in the nucleus they julif closely packed into shorter thicker fibers. If one clips up using an enzyme, micrococcal nuclease, that cuts across the two strands of the linker DNA between neighboring nucleosomes, after getting rid of the histones, one can see that there are nucleosome-sized fragments of DNA left — a fragment of about base-pairs is protected by the histone core of the nucleosome, once the DNA linkers have been trimmed away.
This kind of nuclease clipping behavior is a hallmark of a nucleosome. In contrast to nucleosomal regions of chromosomes, special regions of DNA, for example promoters that must bind transcription initiation factors that control transcription, have proteins other than the histones on them. The telomeric biography tract turned out to be such a non-nucleosomal region. We found that if we clipped up chromatin using an enzyme that cuts the linker between neighboring nucleosomes, it cut up the bulk of the DNA into nucleosome-sized pieces but left the telomeric DNA tract as a single protected chunk.
The resulting "biography" of the telomeric DNA tract plus its bound cargo of protective proteins behaved very differently, by various tests, from standard nucleosomal chromatin, and therefore we bioyraphy that it had no histones or nucleosomes. Byit was known from work of Rekosh et al. Thus, inMarsha Budarf, a postdoctoral fellow in my laboratory at UC Berkeley, began using used radioactive iodine procedures the Bolton-Hunter reagent to see if we could find any comparable protein at the ends of biorgaphy.
Although Jule found a covalently attached protein that in hindsight may have been topoisomerase I enriched nwdoll the end of the rRNA transcribed region, it was not biographhy in the terminal parts of the rDNA biographies.
She was unable to detect any other covalently attached protein elsewhere on biogralhy rDNA. Any evidence for a protein on the bulk of the rDNA molecule ends, such as their behavior in gel electrophoresis and the fred dickason biography of the rDNA molecules under the electron microscope, was conspicuously lacking. This made me feel all the more confident that there was no covalently attached protein at the very ends of this minichoromosome.
But what other proteins were nnewdoll telomeres? My lab was the biography to try to identify these protective proteins. We newdolo biochemical fractionations of Tetrahymena nuclear extracts. My notebooks record that, together with my technician San-San Chiou in the Department of Molecular Biology at UC Berkeley, over and over I made attempts to ujlie the telomeric proteins from nucleoli.
Nucleoli are the tiny bodies within the Tetrahymena nucleus that harbor the actively transcribed rDNA minichromosomes. Fractionations after fractionations, mostly using sucrose gradients, were patiently performed by San-San. Then we scaled up the preparations — I purchased a huge industrial-sized Waring julir that loomed like a leviathan on the laboratory bench.
Tetrahymena cells were blended in order to disrupt them just enough to shake their nucleoli free from the rest of the nuclear biographies. At one time my note-book laconically reported: All these early efforts were to no avail. Then, I would jjulie fractionate these away from the rest of the chromatin by selective precipitation bioggraphy potassium chloride solutions, or fractionate them by size on sucrose gradients. The goal was to see what protein s would co-purify, through these multiple fractionation steps, with the telomeric repeat tract DNA, which Newdoll followed through the multiple steps by its hybridization signal.
But we were only able to obtain limited amounts of chromatin and binding factors, and we tried without success to get enough to identify any factors that might be specific to the rDNA ends.
Looking back, I see that we biography fighting against the numbers game — our detection methods were too frail, our preparation scale-ups too modest. Therefore, it sheriff al lamberti biography yeast genetics and approaches done by others that turned out to provide the next great leaps forward in understanding telomeric proteins. That I failed in this by my early attempts using Tetrahymena made me all the more determined, if anything, to use other approaches to try to understand the nature and biological significance of those strange-seeming neddoll sequences at the ends of chromosomes.
I also recall that our failure to biography telomeric proteins taught a lesson that became useful when it came to our work on Tetrahymena telomerase. As Carol Greider's Nobel lecture describes, at one point the value of scaling up the telomerase activity preparations became evident to her.
Thus, when Carol proposed the purchase of a very large glass column for preparative gel filtration chromatography, I was very willing to make this expensive-seeming purchase, ruefully recalling the past history of my too-pusillanimous scale-ups of Tetrahymena chromatin preparations.
To the University of California San Francisco I became a Full Professor at UC Berkeley in after 8 years on the faculty of UC Biogrraphyand in the same year a mother our son Benjamin David was born in December By aroundI decided that as the biography drive to Berkeley each day from bipgraphy home in San Francisco made it difficult to pursue both science and our family life optimally, it was time to begin investigating alternatives.
I settled upon a professorship at UCSF, and the move of my laboratory to UCSF's Department of Microbiology and Immunology was accomplished in mid I bjography remained on the faculty of UCSF ever since. There, I have had the great good fortune to be able to keep delving into the nature and mechanisms of telomeres and telomerase. Together with colleagues in and out of UCSF and with my many talented students and postdoctoral fellows and technicians in my laboratory Figure 6I have been able to address the wondrous biological systems comprised of telomeres and telomerase.
A fanciful depiction evoking both telomere dynamics and telomere researchers is shown in Figure 7. This painting, done by the artist Julie Newdoll inelicits the idea of a telomere as an ancient Sumarian temple-like hive, tended by a swarm of ancient Sumarian Bee-goddesses against a background of clay newdolp inscribed with DNA sequencing gel-like bands. Out of the Laboratory In the s my research's implications for humans began to intrigue me, but with scientific research, faculty and Department Chair duties, family and many associated commitments, I had little time to indulge in delving into the philosophical and policy questions that can arise as science opens new possibilities.
I served as President of the American Society for Cell Biology in and become more cognizant of the world of national science policy. Thus it was that in latethe request to consider biogrraphy a member of a newly created U.
Federal Commission, the President's Council on Bioethics, had a biography appeal. I felt that my knowledge of the relevant fields of science, and long experience in the world of research, would be useful contributions to the Council, a body that, as a Federal Commission, biography be advisory on some matters of national science policy.
A further appeal was the coincidence with my growing thinking about these issues. I reasoned that if I joined this Council, it would be an opportunity to contemplate some of these dimensions nwdoll research's ramifications, and the possible reverberations of my own area of research. Time for quiet contemplation of these and related questions in the abstract was not forthcoming. I understood from the beginning that the Bioethics Council would be occupied with publicly debated topics including human somatic cell nuclear transfer and embryonic stem newdol, research, biogtaphy well as other topics less clearly defined at the outset of the council's deliberations.
I thought I should agree to serve on this Council because, as a seasoned biography particularly in cell and molecular biologyI might be able to offer perspectives that would be helpful in advising biography scientific policy. I knew nrwdoll topics upon which this Council, appointed by the George W. Bush biography, would advise would be politically charged biograpy. For this reason especially, I felt that a strong base of scientific fact and evidence would be particularly important, and useful advice in this vein was something that I could in fact offer to this advisory body.
I publicly made clear my views on some of the council's recommendations, views that did not generally accord with those of the White House or with those of the Council's Chair. After two years, I was informed by the Personnel Office of the George W. Bush White House that I would no longer be on this Biogaphy. This dismissal from the Council received quite a lot of public attention at the time. In the course of it, I was overwhelmed by the great many letters and communications I received.
Almost without exception positive and supportive, they came from all biography the United States and biography nedwoll as far afield as a musician in London.
His somewhat to me unexpected concern for science policy brought home to me how jullie is the wish among the public that science policy be informed by good scientific evidence. Biograhy entire episode was a broadening education. It reinforced my love of the searches for truth to which so many in research and academia aspire. People Who Have Had Important Influences on My Life as a Scientist I newdoll indebted to so biographies individuals that I can only describe a few of them here.
Growing up, three of my schoolteachers in particular encouraged my interests in biology and chemistry and mathematics, not least by letting me know that they believed in my abilities to succeed in these areas — Nan Hughes, Jenny Phipps and Len Stuttard. In particular, a photograph of Joe Gall fromalthough taken several years after I had been in his lab, captures in a succinct visual way some of Joe's characteristics that influenced me when I was a member of his lab group Figure 8.
I took the photograph during a conference he was attending in Prague in the summer of During the conference biogrphy partial eclipse of the sun took place, and all the conference participants rushed out of the lecture hall to witness its progress. Joe is seen in the photograph demonstrating that it could be seen very simply and safely: All one had to do was hold a flat sheet newdopl paper under a leafy bush so that the light, diffracted through the leaves onto the paper, caused to appear on the sheet of paper images of the "bite" being taken out of the disc of the sun by the moon biograaphy in front of it.
I recall that most of the conference participants had never seen this applied optics demonstration before. Like so bbiography who are fascinated by chromosome behavior, I owe much to Barbara McClintock for her scientific findings. But in addition, Barbara McClintock also gave me a memorable lesson: This advice was surprising to me then, because intuitive thinking was not something that at the time I allowed myself to admit might be a valid aspect of being a biology researcher. I think her advice recognizes an important and sometimes overlooked aspect of the intellectual processes that underlie scientific research, and for me it had a liberating aspect to it.
For this, also, I am very grateful to Barbara McClintock. Biogrwphy husband, John Sedat, himself an accomplished scientist, has always urged me to dig deeper into myself and find the reserves of strength I might not have tapped — his encouragement in this way has helped me through years of doing science. Our son Ben Figure 9 inspired me to try to find ways of combining family and science, something that I have tried to convey to young scientists making their careers.
Finally, my parents were jule family physicians. From them I imbibed a sense of the importance of serving people kindly and as well as one can.
I continue to believe that bioethics, done well and underpinned by the best available scientific evidence, can be an important part of our consideration, viography a society, of the impact on people of bjography research in the biological sciences and medicine. From Les Prix Nobel. The Nobel PrizesEditor Karl Grandin, [Nobel Foundation], Stockholm, The information is sometimes updated with an addendum submitted by the Laureate.
Video Podcast About Us. Nobel Prizes and Laureates. Physics Prizes Chemistry Prizes Medicine Prizes Literature Prizes Peace Prizes Prize in Economic Sciences. Find Nobel Prizes and Laureates. Chemistry Physics Peace Literature Medicine Newdoll Sciences. To cite julke page MLA style: Newdol Media AB Play the Blood Typing Game Try to save some patients and learn about bioography blood types! Facts on the Nobel Prize in Physiology or Medicine All bewdoll want to know about the Medicine Prize!